Biology Internal Assessment Harri Besceli The breakdown of atomic number 1 hydrogen peroxide by catalase Research question: How does temperature affect the browse of answer of the breakdown of hydrogen by catalase? This will be answered by measuring the quantity of oxygen produced over 5 transactions at different temperatures. We can comp argon these results, sexual congress us at which temperatures enzyme activity is at its highest and at which it is at its lowest. The dependant covariant is the quantity of oxygen produced and the independent variable is the temperature. The controlled variables are; pH, enzyme closeness, enzyme volume, come forward area of the enzyme, substrate concentration, substrate volume and time. institution: Enzymes are proteins that act as catalysts. They growing the rate of a reception, but are not utilise up in the reaction themselves. In this experiment, the enzyme catalase is used to fixture up the reaction in which hydrogen peroxide is disconnected down into oxygen and water. This is shown by the symbol equation 2H2O2 2H2O+O2 The four main factors that normally affect the rate of an enzyme reaction are: pH, temperature, substrate concentration and the enzyme concentration.
Apparatus: 2 Fresh Potatoes (of the aforementioned(prenominal) breed) 1 Knife 1 large watertight container (preferably transparent) ~ 2 litre: To hold the water 1 250ml measuring piston chamber 1 water bath 1 rubber fireplug with a hole in and rubber tubes 2 X 6 Test tubes 1 thermometer 1 Stop look fall out man 6 X 50ml Hydrogen Peroxide (30%) 1 cross off of digital Scales 1 Clamp and stand 1 millimeter ruler 1 syringe 1 pair of pair of tongs Met! hod: 1. Cut the white potato vinees using the potato slashedter, and whence cut the piece using a knife to a weighting of 2.5 grams. The pieces should be made rectangular so that the surface areas of the potato pieces are the same. 2. Using a syringe, measure pop out 50ml of hydrogen peroxide into a boiling tube. 3. Fill the tractile container with water to approximately...If you want to get a full essay, command it on our website: OrderEssay.net
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